Selection and Validation of Reference Genes for Pan-Cancer in Platelets Based on RNA-Sequence Data.

ABSTRACT: Many studies in recent years have demonstrated that some messenger RNA (mRNA) in platelets can be used as biomarkers for the diagnosis of pan-cancer. The quantitative real-time polymerase chain reaction (RT-qPCR) molecular technique is most commonly used to determine mRNA expression changes in platelets. Accurate and reliable relative RT-qPCR is highly dependent on reliable reference genes. However, there is no study to validate the reference gene in platelets for pan-cancer. Given that the expression of some commonly used reference genes is altered in certain conditions, selecting and verifying the most suitable reference gene for pan-cancer in platelets is necessary to diagnose early stage cancer. This study performed bioinformatics and functional analysis from the RNA-seq of platelets d

SUBMITTER: Wen X 

PROVIDER: S-EPMC9234127 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature