The Human SCN9A ^R185H Point Mutation Induces Pain Hypersensitivity and Spontaneous Pain in Mice.

ABSTRACT: The voltage-gated sodium channel Nav1.7 is encoded by SCN9A gene and plays a critical role in pain sensitivity. Several SCN9A gain-of-function (GOF) mutations have been found in patients with small fiber neuropathy (SFN) having chronic pain, including the R185H mutation. However, for most of these variants, their involvement in pain phenotype still needs to be experimentally elucidated. In order to delineate the impact of R185H mutation on pain sensitivity, we have established the Scn9a ^R185H mutant mouse model using the CRISPR/Cas9 technology. The Scn9a ^R185H mutant mice show no cellular alteration in the dorsal root ganglia (DRG) containing cell bodies of sensory neurons and no alteration of growth or global he

SUBMITTER: Xue Y 

PROVIDER: S-EPMC9234669 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature