Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles.

ABSTRACT: Skeletal and cardiac muscles are striated myofibers that contain highly organized sarcomeres for muscle contraction. Recent studies revealed that Smyd1, a lysine methyltransferase, plays a key role in sarcomere assembly in heart and trunk skeletal muscles. However, Smyd1 expression and function in craniofacial muscles are not known. Here, we analyze the developmental expression and function of two smyd1 paralogous genes, smyd1a and smyd1b, in craniofacial and cardiac muscles of zebrafish embryos. Our data show that loss of smyd1a (smyd1a^mb5) or smyd1b (smyd1b^sa15678) has no visible effects on myogenic commitment and expression of myod and myosin heavy-chain mRNA transcripts in craniofacial muscles. However, myosin heavy-chain protein accumulation and sarcomere organiz

SUBMITTER: Jiao S 

PROVIDER: S-EPMC9234968 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature