Epigenetic DNA methylation of Zbtb7b regulates the population of double-positive CD4⁺CD8⁺ T cells in ulcerative colitis.

ABSTRACT:

Background and aims

Ulcerative colitis (UC) is a heterogeneous disorder with complex pathogenesis. Therefore, in the present study, we aimed to assess genome-wide DNA methylation changes associated explicitly with the pathogenesis of UC.

Methods

DNA methylation changes were identified by comparing UC tissues with healthy controls (HCs) from the GEO databases. The candidate genes were obtained and verified in clinical samples. Moreover, the underlying molecular mechanism related to Zbtb7b in the pathogenesis of UC was explored using the dextran sodium sulfate (DSS)-induced colitis model.

Results

Bioinformatic analysis from GEO databases confirmed that Zbtb7b, known as Th-inducing POZ-Kruppel factor (ThPOK), was demethylated in UC tissues. Then, we demonstrated that Zbt

SUBMITTER: Xu HM 

PROVIDER: S-EPMC9235105 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature