WTAP-mediated N⁶-methyladenosine modification of NLRP3 mRNA in kidney injury of diabetic nephropathy.

ABSTRACT:

Background

Diabetic nephropathy (DN) is prevalent in patients with diabetes. N⁶-methyladenosine (m⁶A) methylation has been found to cause modification of nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing (NLRP) 3, which is involved in cell pyroptosis and inflammation. WTAP is a key gene in modulating NLRP3 m⁶A.

Methods

In this study, WTAP was silenced or overexpressed in high glucose (HG)-treated HK-2 cells to determine its influence on pyroptosis, NLRP3 inflammasome-related proteins, and the release of pro-inflammatory cytokines. NLRP3 expression and m⁶A levels were assessed in the presence of WTAP shRNA (shWTAP). WTAP expression in HK-2 cells was examined with the introduction of C646, a

SUBMITTER: Lan J 

PROVIDER: S-EPMC9235192 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature