Astrocyte reactivation in medial prefrontal cortex contributes to obesity-promoted depressive-like behaviors.

ABSTRACT:

Background

Little is known about how the obesogenic environment influences emotional states associated with glial responses and neuronal function. Here, we investigated glial reactivation and neuronal electrophysiological properties in emotion-related brain regions of high-fat diet (HFD) and ob/ob mice under chronic stress.

Methods

The glial reactivation and neuronal activities in emotion-related brain regions were analyzed among normal diet mice (ND), HFD mice, wild-type mice, and ob/ob mice. To further activate or inhibit astrocytes in medial prefrontal cortex (mPFC), we injected astrocytes specific Gq-AAV or Gi-AAV into mPFC and ongoing treated mice with CNO.

Results

The results showed that obesogenic factors per se had no significant effect on neuronal activities

SUBMITTER: Yu G 

PROVIDER: S-EPMC9235218 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature