Succession of pelagic marine bacteria during enrichment: a close look at cultivation-induced shifts.

ABSTRACT: Enrichment experiments with North Sea bacterioplankton were performed to test if rapid incubation-induced changes in community structure explain the frequent isolation of members of a few particular bacterial lineages or if readily culturable bacteria are common in the plankton but in a state of dormancy. A metabolic inhibitor of cell division (nalidixic acid [NA]) was added to substrate-amended (S+) and unamended (S-) grazer-free seawater samples, and shifts in community composition and per cell DNA and protein content were compared with untreated controls. In addition, starvation survival experiments were performed on selected isolates. Incubations resulted in rapid community shifts towards typical culturable genera rather than in the activation of either dormant cells or the original DN

SUBMITTER: Eilers H 

PROVIDER: S-EPMC92360 | biostudies-literature | 2000 Nov

REPOSITORIES: biostudies-literature