A biosensing system employing nanowell microelectrode arrays to record the intracellular potential of a single cardiomyocyte.

ABSTRACT: Electrophysiological recording is a widely used method to investigate cardiovascular pathology, pharmacology and developmental biology. Microelectrode arrays record the electrical potential of cells in a minimally invasive and high-throughput way. However, commonly used microelectrode arrays primarily employ planar microelectrodes and cannot work in applications that require a recording of the intracellular action potential of a single cell. In this study, we proposed a novel measuring method that is able to record the intracellular action potential of a single cardiomyocyte by using a nanowell patterned microelectrode array (NWMEA). The NWMEA consists of five nanoscale wells at the center of each circular planar microelectrode. Biphasic pulse electroporation was applied to the NWMEA to pe

SUBMITTER: Xiang Y 

PROVIDER: S-EPMC9237042 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature