Heterologous expression of cry3Bb1 and cry3 genes for enhanced resistance against insect pests in cotton.

ABSTRACT: Transgenic technology played a crucial role in developing insect-resistant plants resulting in the reduced application of pesticides. This article reports the expression of two cry proteins (Cry3Bb1 and Cry3) in cotton for enhanced resistance against chewing insect pests. The aforementioned genes were synthetically developed and were cloned under appropriate regulatory sequences followed by transformation into Eagle-2 genotype (Gossypium hirsutum) of cotton through shoot apex-cut Agro-infiltration. The transgene integration was validated by polymerase chain reaction using primers flanking the aforementioned cry genes. Transgene expression was assessed by qRT-PCR using GADPH as a reference gene. The relative fold expression analyses revealed the highest expression of the transgene(s) in M1

SUBMITTER: Zafar MM 

PROVIDER: S-EPMC9237104 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature