METTL3-Mediated m⁶A RNA Methylation of ZBTB4 Interferes With Trophoblast Invasion and Maybe Involved in RSA.

ABSTRACT: N⁶-methyladenosine (m⁶A) was the most abundant modification of mRNA and lncRNA in mammalian cells and played an important role in many biological processes. However, whether m⁶A modification was associated with recurrent spontaneous abortion (RSA) and its roles were still unclear. Methods: Methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was used to study the global m⁶A modification pattern in RSAs and controls. RNA sequencing (RNA-Seq) was used to study the level of global mRNA in two groups. Real-time quantitative PCR (RT-qPCR) was used to verify the level of mRNA of METTL3 and ZBTB4. MeRIP-qPCR was conducted to test the level of ZBTB4 m⁶A modification in two groups. In order to further explore whether ZBTB4 was the s

SUBMITTER: Huang N 

PROVIDER: S-EPMC9237410 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature