Differential Gene Expression and Withanolides Biosynthesis During in vitro and ex vitro Growth of Withania somnifera (L.) Dunal.

ABSTRACT: Ashwagandha (Withania somnifera L. Dunal) is a medicinally important plant with withanolides as its major bioactive compounds, abundant in the roots and leaves. We examined the influence of plant growth regulators (PGRs) on direct organogenesis, adventitious root development, withanolide biosynthetic pathway gene expression, withanolide contents, and metabolites during vegetative and reproductive growth phases under in vitro and ex vitro conditions. The highest shooting responses were observed with 6-benzylaminopurine (BAP) (2.0 mg L^-1) + Kinetin (KIN) (1.5 mg L^-1) supplementation. Furthermore, BAP (2.0 mg L^-1) + KIN (1.5 mg L^-1) + gibberellic acid (GA₃) (0.5 mg L^-1) exhibited better elongation responses

SUBMITTER: Thorat SA 

PROVIDER: S-EPMC9237602 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature