Decreased Kruppel-like factor 4 in adenomyosis impairs decidualization by repressing autophagy in human endometrial stromal cells.

ABSTRACT:

Background

Poor decidualization and abnormal autophagy conditions in the endometria of adenomyosis patients have been reported previously. However, the specific regulatory mechanism of decidualization in adenomyosis and its relationship with autophagy levels have not been clarified.

Methods

Endometrial tissues from adenomyosis patients and uteri from an adenomyosis mouse model were collected for the detection of different expression patterns of KLF4 and autophagy markers (LC3-B/LC3-A and Beclin-1) compared with control groups. Human endometrial stromal cells (hESCs) isolated from adenomyosis and control endometrial tissues were employed to elucidate the biological functions of KLF4 in autophagy and decidualization. Gene expression regulation was examined by quantitative real

SUBMITTER: Mei J 

PROVIDER: S-EPMC9238063 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature