Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins.

ABSTRACT: Transition metals such as zinc and copper are essential in numerous life processes, and both deficiency and toxic overload of these metals are associated with various diseases. Fluorescent metal sensors are powerful tools for studying the roles of metal ions in the physiology and pathology of biological systems. Green fluorescent protein (GFP) and its derivatives are highly utilized for protein-based sensor design, but application to anaerobic systems is limited because these proteins require oxygen to become fluorescent. Bacteriophytochrome-based monomeric near-infrared fluorescent proteins (miRFPs) covalently bind a bilin cofactor, which can be added exogenously for anaerobic cells. miRFPs can also have emission wavelengths extending to >700 nm, which is valuable for imaging applications

SUBMITTER: Zhao H 

PROVIDER: S-EPMC9238257 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature