Raman microspectroscopy fingerprinting of organoid differentiation state.

ABSTRACT:

Background

Organoids, which are organs grown in a dish from stem or progenitor cells, model the structure and function of organs and can be used to define molecular events during organ formation, model human disease, assess drug responses, and perform grafting in vivo for regenerative medicine approaches. For therapeutic applications, there is a need for nondestructive methods to identify the differentiation state of unlabeled organoids in response to treatment with growth factors or pharmacologicals.

Methods

Using complex 3D submandibular salivary gland organoids developed from embryonic progenitor cells, which respond to EGF by proliferating and FGF2 by undergoing branching morphogenesis and proacinar differentiation, we developed Raman confocal microspectroscopy methods t

SUBMITTER: Tubbesing K 

PROVIDER: S-EPMC9238268 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature