Dynamic change of fecal microbiota and metabolomics in a polymicrobial murine sepsis model.

ABSTRACT:

Aim

Sepsis causes a systemic inflammatory reaction by destroying intestinal flora, which leads to a poor prognosis. In this study, we sought to clarify the characteristics of fecal flora and metabolites in a mouse model of sepsis by comprehensive metagenomic and metabolomic analysis.

Methods

We performed a cecal ligation and puncture model procedure to create a mild sepsis model. We collected fecal samples on day 0 (healthy condition) and days 1 and 7 after the cecal ligation and puncture to determine the microbiome and metabolites. We analyzed fecal flora using 16S rRNA gene sequencing and metabolites using capillary electrophoresis mass spectrometry with time-of-flight analysis.

Results

The abundance of bacteria belonging to the family Enterobacteriaceae sign

SUBMITTER: Muratsu A 

PROVIDER: S-EPMC9238289 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature