Prior Freezing Has Minimal Impact on the Contractile Properties of Permeabilized Human Myocardium.

ABSTRACT: Background Experiments measuring the contractile properties of human myocardium are important for translational research but complicated by the logistical difficulties of acquiring specimens. Accordingly, many groups perform contractile assays using samples that are acquired from patients at one institution and shipped to another institution for experiments. This necessitates freezing the samples and performing subsequent assays using chemically permeabilized preparations. It is unknown how prior freezing affects the contractile function of these preparations. Methods and Results To examine the effects of freezing we measured the contractile function of never-frozen and previously frozen myocardial samples. Samples of left ventricular tissue were obtained from 7 patients who were having a

SUBMITTER: Milburn GN 

PROVIDER: S-EPMC9238558 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature