Regulation of Translation by Lysine Acetylation in Escherichia coli.

ABSTRACT: Nε-lysine acetylation is a common posttranslational modification observed in diverse species of bacteria. Aside from a few central metabolic enzymes and transcription factors, little is known about how this posttranslational modification regulates protein activity. In this work, we investigated how lysine acetylation affects translation in Escherichia coli. In multiple species of bacteria, ribosomal proteins are highly acetylated at conserved lysine residues, suggesting that this modification may regulate translation. In support of this hypothesis, we found that the addition of either of the acetyl donors acetyl phosphate and acetyl-coenzyme A inhibits translation but not transcription using an E. coli cell-free system. Further investigations using in vivo assays revealed tha

SUBMITTER: Feid SC 

PROVIDER: S-EPMC9239087 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature