A Dual Enrichment Strategy Provides Soil- and Digestate-Competent Nitrous Oxide-Respiring Bacteria for Mitigating Climate Forcing in Agriculture.

ABSTRACT: Manipulating soil metabolism through heavy inoculation with microbes is feasible if organic wastes can be utilized as the substrate for growth and vector as a fertilizer. This, however, requires organisms active in both digestate and soil (generalists). Here, we present a dual enrichment strategy to enrich and isolate such generalists among N₂O-respiring bacteria (NRB) in soil and digestates, to be used as an inoculum for strengthening the N₂O-reduction capacity of soils. The enrichment strategy utilizes sequential batch enrichment cultures alternating between sterilized digestate and soil as substrates, with each batch initiated with limited O₂ and unlimited N₂O. The cultures were monitored for gas kinetics and community composition. As predicte

SUBMITTER: Jonassen KR 

PROVIDER: S-EPMC9239227 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature