Bcar1/p130Cas is essential for ventricular development and neural crest cell remodelling of the cardiac outflow tract.

ABSTRACT:

Aims

The adapter protein p130Cas, encoded by the Bcar1 gene, is a key regulator of cell movement, adhesion, and cell cycle control in diverse cell types. Bcar1 constitutive knockout mice are embryonic lethal by embryonic days (E) 11.5-12.5, but the role of Bcar1 in embryonic development remains unclear. Here, we investigated the role of Bcar1 specifically in cardiovascular development and defined the cellular and molecular mechanisms disrupted following targeted Bcar1 deletions.

Methods and results

We crossed Bcar1 floxed mice with Cre transgenic lines allowing for cell-specific knockout either in smooth muscle and early cardiac tissues (SM22-Cre), mature smooth muscle cells (smMHC-Cre), endothelial cells (Tie2-Cre), second heart field cells (Mef2c-Cre), or neural crest cell

SUBMITTER: Mahmoud M 

PROVIDER: S-EPMC9239580 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature