Subclinical infection occurs frequently following low dose exposure to prions by blood transfusion.

ABSTRACT: Infectious prion diseases have very long incubation periods, and the role that subclinical infections play in transmission, persistence and re-emergence of these diseases is unclear. In this study, we used a well-established model of vCJD (sheep experimentally infected with bovine spongiform encephalopathy, BSE) to determine the prevalence of subclinical infection following exposure by blood transfusion from infected donors. Many recipient sheep survived for years post-transfusion with no clinical signs and no disease-associated PrP (PrP^Sc) found in post mortem tissue samples by conventional tests. Using a sensitive protein misfolding cyclic amplification assay (PMCA), we found that the majority of these sheep had detectable PrP^Sc in lymph node samples, at levels appr

SUBMITTER: Salamat MKF 

PROVIDER: S-EPMC9240018 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature