Cell fate determining molecular switches and signaling pathways in Pax7-expressing somitic mesoderm.

ABSTRACT: During development, different cell types originate from a common progenitor at well-defined time points. Previous lineage-tracing of Pax7⁺ progenitors from the somitic mesoderm has established its developmental trajectory towards the dermis, brown adipocytes, and skeletal muscle in the dorsal trunk; yet the molecular switches and mechanisms guiding the differentiation into different lineages remain unknown. We performed lineage-tracing of Pax7-expressing cells in mouse embryos at E9.5 and profiled the transcriptomes of Pax7-progenies on E12.5, E14.5, and E16.5 at single-cell level. Analysis of single-cell transcriptomic data at multiple time points showed temporal-specific differentiation events toward muscle, dermis, and brown adipocyte, identified marker genes for putative pro

SUBMITTER: Fung CW 

PROVIDER: S-EPMC9240041 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature