Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC.

ABSTRACT: The purpose of this study was to investigate the structural characterization and immunological activity in vitro and in vivo of a polysaccharide from the rhizome of Menispermum dauricum. A new polysaccharide named MDP was isolated from the rhizome of Menispermum dauricum by hot water extraction, ethanol precipitation, anion-exchange, and gel-filtration chromatography. MDP was homogeneous and had a molecular weight of 6.16 ×10³ Da, and it was an α-D-glucan containing a (1 → 6)-linked backbone, with a glucosyl residue at the C-3 position along the main chain. MDP exhibited immunological activity in vitro, which could significantly promote the proliferation and phagocytosis of RAW264.7 cells and the release of TNF-α and IL-6 factors. For immunolog

SUBMITTER: Yang P 

PROVIDER: S-EPMC9240474 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature