Microbial Tracking-2, a metagenomics analysis of bacteria and fungi onboard the International Space Station.

ABSTRACT:

Background

The International Space Station (ISS) is a unique and complex built environment with the ISS surface microbiome originating from crew and cargo or from life support recirculation in an almost entirely closed system. The Microbial Tracking 1 (MT-1) project was the first ISS environmental surface study to report on the metagenome profiles without using whole-genome amplification. The study surveyed the microbial communities from eight surfaces over a 14-month period. The Microbial Tracking 2 (MT-2) project aimed to continue the work of MT-1, sampling an additional four flights from the same locations, over another 14 months.

Methods

Eight surfaces across the ISS were sampled with sterile wipes and processed upon return to Earth. DNA extracted from the processed samp

SUBMITTER: Urbaniak C 

PROVIDER: S-EPMC9241228 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature