HrpA anchors meningococci to the dynein motor and affects the balance between apoptosis and pyroptosis.

ABSTRACT:

Background

In Neisseria meningitidis the HrpA/HrpB two-partner secretion system (TPS) was implicated in diverse functions including meningococcal competition, biofilm formation, adherence to epithelial cells, intracellular survival and vacuolar escape. These diverse functions could be attributed to distinct domains of secreted HrpA.

Methods

A yeast two-hybrid screening, in vitro pull-down assay and immunofluorescence microscopy experiments were used to investigate the interaction between HrpA and the dynein light-chain, Tctex-type 1 (DYNLT1). In silico modeling was used to analyze HrpA structure. Western blot analysis was used to investigate apoptotic and pyroptotic markers.

Results

The HrpA carboxy-terminal region acts as a manganese-dependent cell lysin, while the r

SUBMITTER: Tala A 

PROVIDER: S-EPMC9241232 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature