Enhanced angiogenic potential of adipose-derived stem cell sheets by integration with cell spheroids of the same source.

ABSTRACT:

Background

Adipose-derived stem cell (ASC) has been considered as a desirable source for cell therapy. In contrast to combining scaffold materials with cells, ASCs can be fabricated into scaffold-free three-dimensional (3D) constructs to promote regeneration at tissue level. However, previous reports have found decreased expression of vascular endothelial growth factor (VEGF) in ASC sheets. In this study, we aimed to integrate ASC spheroids into ASC sheets to enhance the angiogenic capability of cell sheets.

Methods

ASCs were seeded in agarose microwells to generate uniform cell spheroids with adjustable size, while extracellular matrix deposition could be stimulated by ascorbic acid 2-phosphate to form ASC sheets. RNA sequencing was performed to identify the transcriptomic

SUBMITTER: Yu J 

PROVIDER: S-EPMC9241243 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature