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Genomes of single- and double-petal jasmines (Jasminum sambac) provide insights into their divergence time and structural variations.

ABSTRACT:

SUBMITTER: Wang P

PROVIDER: S-EPMC9241369 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Genomes of single- and double-petal jasmines (Jasminum sambac) provide insights into their divergence time and structural variations.

Wang Pengjie P Fang Jingping J Lin Hongzheng H Yang Wenwen W Yu Jiaxin J Hong Yaping Y Jiang Mengwei M Gu Mengya M Chen Qinchang Q Zheng Yucheng Y Liao ZhenYang Z Chen Guixin G Yang Jiangfan J Jin Shan S Zhang Xingtan X Ye Naixing N

Plant biotechnology journal 20220427 7

PMID: 35373460

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Project description:BackgroundJasminum sambac, a widely recognized ornamental plant prized for its aromatic blossoms, exhibits three flora phenotypes: single-petal ("SP"), double-petal ("DP"), and multi-petal ("MP"). The lack of detailed characterization and comparison of J. sambac mitochondrial genomes (mitogenomes) hinders the exploration of the genetic and structural diversity underlying the varying floral phenotypes in jasmine accessions.ResultsHere, we de novo assembled three mitogenomes of typical phenotypes of J. sambac, "SP", "DP", and "MP-hutou" ("HT"), with PacBio reads and the "HT" chloroplast (cp) genome with Illumina reads, and verified them with read mapping and fluorescence in situ hybridization (FISH). The three mitogenomes present divergent sub-genomic conformations, with two, two, and four autonomous circular chromosomes ranging in size from 35.7 kb to 405.3 kb. Each mitogenome contained 58 unique genes. Ribosome binding sites with conserved AAGAAx/AxAAAG motifs were detected upstream of uncanonical start codons TTG, CTG and GTG. The three mitogenomes were similar in genomic content but divergent in structure. The structural variations were mainly attributed to recombination mediated by a large (~ 5 kb) forward repeat pair and several short repeats. The three jasmine cp. genomes showed a well-conserved structure, apart from a 19.9 kb inversion in "HT". We identified a 14.3 kb "HT"-specific insertion on Chr7 of the "HT" nuclear genome, consisting of two 7 kb chloroplast-derived fragments with two intact ndhH and rps15 genes, further validated by polymerase chain reaction (PCR). The well-resolved phylogeny suggests faster mitogenome evolution in J. sambac compared to other Oleaceae species and outlines the mitogenome evolutionary trajectories within Lamiales. All evidence supports that "DP" and "HT" evolved from "SP", with "HT" being the most recent derivative of "DP".ConclusionThe comprehensive characterization of jasmine organelle genomes has added to our knowledge of the structural diversity and evolutionary trajectories behind varying jasmine traits, paving the way for in-depth exploration of mechanisms and targeted genetic research.

Dataset Information

Genomes of single- and double-petal jasmines (Jasminum sambac) provide insights into their divergence time and structural variations.

Publications

Genomes of single- and double-petal jasmines (Jasminum sambac) provide insights into their divergence time and structural variations.

Similar Datasets

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