A High-Throughput Yellow Fever Neutralization Assay.

ABSTRACT: Quick and accurate detection of neutralizing antibodies (nAbs) against yellow fever is essential in serodiagnosis during outbreaks for surveillance and to evaluate vaccine efficacy in population-wide studies. All of this requires serological assays that can process a large number of samples in a highly standardized format. Albeit being laborious, time-consuming, and limited in throughput, the classical plaque reduction neutralization test (PRNT) is still considered the gold standard for the detection and quantification of nAbs due to its sensitivity and specificity. Here, we report the development of an alternative fluorescence-based serological assay (SNT^FLUO) with an equally high sensitivity and specificity that is fit for high-throughput testing with the potential for automat

SUBMITTER: Rasulova M 

PROVIDER: S-EPMC9241659 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature