Prophylactic Delivery of a Bacteriophage Cocktail in Feed Significantly Reduces Salmonella Colonization in Pigs.

ABSTRACT: Nontyphoidal Salmonella spp. are a leading cause of human food poisoning and can be transmitted to humans via consuming contaminated pork. To reduce Salmonella spread to the human food chain, bacteriophage (phage) therapy could be used to reduce bacteria from animals' preslaughter. We aimed to determine if adding a two-phage cocktail to feed reduces Salmonella colonization in piglets. This first required spray drying phages to allow them to be added as a powder to feed, and phages were spray dried in different excipients to establish maximum recovery. Although laboratory phage yields were not maintained during scale up in a commercial spray dryer (titers fell from 3 × 10⁸ to 2.4 × 10⁶ PFU/g respectively), the phage titers were high enough to progress. Spray dried phag

SUBMITTER: Thanki AM 

PROVIDER: S-EPMC9241700 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature