Spermidine Is an Intercellular Signal Modulating T3SS Expression in Pseudomonas aeruginosa.

ABSTRACT: Pseudomonas aeruginosa is a vital opportunistic human bacterial pathogen that causes acute and chronic infections. In this study, we set to determine whether the endogenous spermidine biosynthesis plays a role in regulation of type III secretion system (T3SS). The results showed that deletion of speA and speC, which encode putrescine biosynthesis, did not seem to affect cellular spermidine level and the T3SS gene expression. In contrast, mutation of speD and speE encoding spermidine biosynthesis led to significantly decreased spermidine production and expression of T3SS genes. We also showed that endogenous spermidine could auto-induce the transcriptional expression of speE and its full functionality required the transporter SpuDEFGH. Cytotoxicity analysi

SUBMITTER: Lin Q 

PROVIDER: S-EPMC9241758 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature