Three-Dimensional Genome Map of the Filamentous Fungus Penicillium oxalicum.

ABSTRACT: Higher-order spatial organization of the chromatin in the nucleus plays crucial roles in the maintenance of cell functions and the regulation of gene expression. Three-dimensional (3D) genome sequencing has been used to great effect in mammal and plants, but the availability of 3D genomes of filamentous fungi is severely limited. Here, we performed a chromosome-level genome assembly of Penicillium oxalicum through single-molecule real-time sequencing (Pacific Biosciences) and chromatin interaction mapping (Hi-C), with a scaffold N₅₀ of 4.07 Mb and a contig N₅₀ of 3.81 Mb, and further elucidated the 3D genome architecture of P. oxalicum. High-frequency interchromosomal contacts occurred within the centromeres and telomeres, as well as within individual ch

SUBMITTER: Li CX 

PROVIDER: S-EPMC9241887 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature