DWV 3C Protease Uncovers the Diverse Catalytic Triad in Insect RNA Viruses.

ABSTRACT: Deformed wing virus (DWV) is the most prevalent Iflavirus that is infecting honey bees worldwide. However, the mechanisms of its infection and replication in host cells are poorly understood. In this study, we analyzed the structure and function of DWV 3C protease (3C^pro), which is necessary for the cleavage of the polyprotein to synthesize mature viral proteins. Thus, it is one of the nonstructural viral proteins essential for the replication. We found that the 3C^pros of DWV and picornaviruses share common enzymatic properties, including sensitivity to the same inhibitors, such as rupintrivir. The predicted structure of DWV 3C^pro by AlphaFold2, the predicted rupintrivir binding domain, and the protease activities of mutant proteins revealed that it has a C

SUBMITTER: Yuan X 

PROVIDER: S-EPMC9241925 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature