Biocatalytic cascades and intercommunicated biocatalytic cascades in microcapsule systems.

ABSTRACT: Biomolecule-loaded nucleic acid-functionalized carboxymethyl cellulose hydrogel-stabilized microcapsules (diameter ca. 2 μm) are introduced as cell-like containments. The microcapsules are loaded with two DNA tetrahedra, T₁ and T₂, functionalized with guanosine-rich G-quadruplex subunits, and/or with native enzymes (glucose oxidase, GOx, and/or β-galactosidase, β-gal). In the presence of K⁺-ions and hemin, the T₁/T₂ tetrahedra constituents, loaded in the microcapsules, assemble into a hemin/G-quadruplex bridged tetrahedra dimer DNAzyme catalyzing the oxidation of Amplex Red to Resorufin by generating H₂O₂. In the presence of co-loaded GOx or GOx/β-gal, the GOx//T₁/T₂ hemin/G-quadruplex

SUBMITTER: Zhang P 

PROVIDER: S-EPMC9241983 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature