Peptidoglycan-Like Components in Z-100, Extracted from Mycobacterium tuberculosis Strain Aoyama B, Increase IL-12p40 via NOD2.

ABSTRACT:

Background

Z-100 is a hot-water extract of the human-type Mycobacterium tuberculosis strain Aoyama B. While Z-100's macrophage-mediated immunomodulatory effects have been reported, the mechanistic details have not been fully clarified. Here, we studied the immunomodulatory effects of Z-100 on mouse bone marrow-derived cells, human CD14⁺ cells, and skin.

Methods

Mouse bone marrow-derived cells and CD14⁺ cells were cultured in the presence of granulocyte-macrophage colony-stimulating factor, differentiated into macrophage-like cells, and then stimulated with Z-100. Furthermore, since Z-100 is subcutaneously administered clinically, we injected Z-100 into mice and measured gene expression in the skin.

Results

While Z-100 stimulation increased

SUBMITTER: Horii T 

PROVIDER: S-EPMC9242757 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature