Cryogenic infrared spectroscopy provides mechanistic insight into the fragmentation of phospholipid silver adducts.

ABSTRACT: Tandem mass spectrometry is arguably the most important analytical tool for structure elucidation of lipids and other metabolites. By fragmenting intact lipid ions, valuable structural information such as the lipid class and fatty acyl composition are readily obtainable. The information content of a fragment spectrum can often be increased by the addition of metal cations. In particular, the use of silver ions is deeply rooted in the history of lipidomics due to their propensity to coordinate both electron-rich heteroatoms and C = C bonds in aliphatic chains. Not surprisingly, coordination of silver ions was found to enable the distinction of sn-isomers in glycerolipids by inducing reproducible intensity differences in the fragment spectra, which could, however, not be rationalized. Here,

SUBMITTER: Kirschbaum C 

PROVIDER: S-EPMC9242943 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature