FFF-based high-throughput sequence shortlisting to support the development of aptamer-based analytical strategies.

ABSTRACT: Aptamers are biomimetic receptors that are increasingly exploited for the development of optical and electrochemical aptasensors. They are selected in vitro by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure, but although they are promising recognition elements, for their reliable applicability for analytical purposes, one cannot ignore sample components that cause matrix effects. This particularly applies when different SELEX-selected aptamers and related truncated sequences are available for a certain target, and the choice of the aptamer should be driven by the specific downstream application. In this context, the present work aimed at investigating the potentialities of asymmetrical flow field-flow fractionation (AF4) with UV detection for the developmen

SUBMITTER: Marassi V 

PROVIDER: S-EPMC9242963 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature