A quartz crystal microbalance method to quantify the size of hyaluronan and other glycosaminoglycans on surfaces.

ABSTRACT: Hyaluronan (HA) is a major component of peri- and extra-cellular matrices and plays important roles in many biological processes such as cell adhesion, proliferation and migration. The abundance, size distribution and presentation of HA dictate its biological effects and are also useful indicators of pathologies and disease progression. Methods to assess the molecular mass of free-floating HA and other glycosaminoglycans (GAGs) are well established. In many biological and technological settings, however, GAGs are displayed on surfaces, and methods to obtain the size of surface-attached GAGs are lacking. Here, we present a method to size HA that is end-attached to surfaces. The method is based on the quartz crystal microbalance with dissipation monitoring (QCM-D) and exploits that the softn

SUBMITTER: Srimasorn S 

PROVIDER: S-EPMC9243130 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature