Functional imaging with dynamic quantitative oblique back-illumination microscopy.

ABSTRACT:

Significance

Quantitative oblique back-illumination microscopy (qOBM) is a recently developed label-free imaging technique that enables 3D quantitative phase imaging of thick scattering samples with epi-illumination. Here, we propose dynamic qOBM to achieve functional imaging based on subcellular dynamics, potentially indicative of metabolic activity. We show the potential utility of this novel technique by imaging adherent mesenchymal stromal cells (MSCs) grown in bioreactors, which can help address important unmet needs in cell manufacturing for therapeutics.

Aim

We aim to develop dynamic qOBM and demonstrate its potential for functional imaging based on cellular and subcellular dynamics.

Approach

To obtain functional images with dynamic qOBM, a sample is imaged ove

SUBMITTER: Casteleiro Costa P 

PROVIDER: S-EPMC9243522 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature