Production and Purification of Phosphatidylinositol Mannosides from Mycobacterium smegmatis Biomass.

ABSTRACT: Mycobacterium tuberculosis, the etiological agent of tuberculosis, is regarded as the most successful pathogen of humankind and a major threat to global health. The mycobacterial cell wall is vital for cell growth, virulence, and resistance to antibiotics, and thus constitutes a unique target for drug development. To characterize the enzymes catalyzing the synthesis of the cell wall components, considerable amounts of substrates are required. Since many mycobacterial cell wall lipids, particularly phosphatidylinositol mannosides (PIMs), are not commercially available, isolation from cell biomass is the most straightforward way to obtain these compounds. In this study, we optimized a protocol to extract and purify PIM species, in particular Ac₁ PIM₂ and Ac₁

SUBMITTER: Nobre RN 

PROVIDER: S-EPMC9245178 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature