CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m⁶A-modified CREB1 mRNA.

ABSTRACT:

Background

Aberrant expression of circular RNAs (circRNAs) contributes to the initiation and progression of human malignancies, but the underlying mechanisms remain largely elusive.

Methods

High-throughput sequencing was performed to screen aberrantly expressed circRNAs or miRNAs in colorectal cancer (CRC) and adjacent normal tissues. A series of gain- and loss-of-function studies were conducted to evaluate the biological behaviors of CRC cells. RNA pulldown, mass spectrometry, RIP, qRT-PCR, Western blot, luciferase reporter assays and MeRIP-seq analysis were further applied to dissect the detailed mechanisms.

Results

Here, a novel circRNA named circEZH2 (hsa_circ_0006357) was screened out by RNA-seq in CRC tissues, whose expression is closely related to the clinicpat

SUBMITTER: Yao B 

PROVIDER: S-EPMC9245290 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature