Pore-Opening Dynamics of Single Nanometer Biovesicles at an Electrified Interface.

ABSTRACT: Release from nanobiovesicles via a pore generated by membrane electroporation at an electrified interface can be monitored by vesicle impact electrochemical cytometry (VIEC) and provides rich information about the various vesicular content transfer processes, including content homeostasis, intraphase content transfer, or the transient fusion of vesicles. These processes are primarily influenced by the vesicular pore-opening dynamics at the electrified interface which has not been disclosed at the single nanobiovesicle level yet. In this work, after simultaneously measuring the size and release dynamics of individual vesicles, we employed a moving mesh-finite element simulation algorithm to reconstruct the accurate pore-opening dynamics of individual vesicles with different sizes during VIE

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9245343 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature