Flow-FISH as a Tool for Studying Bacteria, Fungi and Viruses.

ABSTRACT: Many techniques are currently in use to study microbes. These can be aimed at detecting, identifying, and characterizing bacterial, fungal, and viral species. One technique that is suitable for high-throughput analysis is flow cytometry-based fluorescence in situ hybridization, or Flow-FISH. This technique employs (fluorescently labeled) probes directed against DNA or (m)RNA, for instance targeting a gene or microorganism of interest and provides information on a single-cell level. Furthermore, by combining Flow-FISH with antibody-based protein detection, proteins of interest can be measured simultaneously with genetic material. Additionally, depending on the type of Flow-FISH assay, Flow-FISH can also be multiplexed, allowing for the simultaneous measurement of multiple gene targets and/o

SUBMITTER: Freen-van Heeren JJ 

PROVIDER: S-EPMC9245478 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature