Cell-Type-Specific Circadian Bioluminescence Rhythms in <i>Dbp</i> Reporter Mice.

ABSTRACT: Circadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report development of a reporter mouse generated by modification of a widely expressed and highly rhythmic gene encoding D-site albumin promoter binding protein (Dbp). In this line of mice, firefly luciferase is expressed from the Dbp locus in a Cre recombinase-dependent manner, allowing assessment of bioluminescence rhythms in specific cellular populations. A mouse line in which luciferase expression was Cre-independent was also generated. The Dbp reporter alleles do not alter Dbp gene expression rhythms in liver or ci

SUBMITTER: Smith CB 

PROVIDER: S-EPMC9245534 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature