SRS-FISH: A high-throughput platform linking microbiome metabolism to identity at the single-cell level.

ABSTRACT: One of the biggest challenges in microbiome research in environmental and medical samples is to better understand functional properties of microbial community members at a single-cell level. Single-cell isotope probing has become a key tool for this purpose, but the current detection methods for determination of isotope incorporation into single cells do not allow high-throughput analyses. Here, we report on the development of an imaging-based approach termed stimulated Raman scattering-two-photon fluorescence in situ hybridization (SRS-FISH) for high-throughput metabolism and identity analyses of microbial communities with single-cell resolution. SRS-FISH offers an imaging speed of 10 to 100 ms per cell, which is two to three orders of magnitude faster than achievable by state-of-the-art

SUBMITTER: Ge X 

PROVIDER: S-EPMC9245642 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature