Tas2R activation relaxes airway smooth muscle by release of Gα_t targeting on AChR signaling.

ABSTRACT: Both chronic obstructive pulmonary disease (COPD) and asthma are severe respiratory diseases. Bitter receptor-mediated bronchodilation is a potential therapy for asthma, but the mechanism underlying the agonistic relaxation of airway smooth muscle (ASM) is not well defined. By exploring the ASM relaxation mechanism of bitter substances, we observed that pretreatment with the bitter substances nearly abolished the methacholine (MCh)-induced increase in the ASM cell (ASMC) calcium concentration, thereby suppressing the calcium-induced contraction release. The ASM relaxation was significantly inhibited by simultaneous deletion of three Gα_t proteins, suggesting an interaction between Tas2R and AChR signaling cascades in the relaxation process. Biochemically, the Gα_t relea

SUBMITTER: Zhou YW 

PROVIDER: S-EPMC9245679 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature