The Identification of Ethidium Bromide-Degrading Bacteria from Laboratory Gel Electrophoresis Waste.

ABSTRACT: Ethidium bromide (EtBr) is widely used in most laboratories to detect nucleic acids in gel electrophoresis applications. It is a well-known carcinogenic and mutagenic agent, which can affect biotic components of the place in which it is disposed. Usually the gel-waste is either buried in the ground or incinerated, whereas the liquid waste is disposed of down the sink following the recommended methods of treatment. The recommended methods do not involve biological potential, but rather make use of chemicals, which may further deteriorate soil and water quality. The present study identifies and characterizes the EtBr-degrading bacterial isolates BR3 and BR4. A bibliographic review of the risk status of using these isolates for the treatment of lab waste in laboratory settings is also present

SUBMITTER: Gandhi VP 

PROVIDER: S-EPMC9245905 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature