Metagenomic Insights into the Composition and Function of Microbes Associated with the Rootzone of Datura inoxia.

ABSTRACT: The purpose of this paper is to elucidate the roles that microbes may be playing in the rootzone of the medicinal plant Daturainoxia. We hypothesized that the microbes associated with the Datura rootzone would be significantly different than the similar surrounding fields in composition and function. We also hypothesized that rhizospheric and endophytic microbes would be associated with similar metabolic functions to the plant rootzone they inhabited. The methods employed were microbial barcoding, tests of essential oils against antibiotic resistant bacteria and other soil bacterial isolates, 16S Next Generation Sequencing (NGS) metabarcoding, and Whole Genome Shotgun (WGS) taxonomic and functional analyses. A few of the main bacterial genera of interest that were diff

SUBMITTER: Senn S 

PROVIDER: S-EPMC9245906 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature