Glial Cell Expansion Coincides with Neural Circuit Formation in the Developing Auditory Brainstem.

ABSTRACT: Neural circuit formation involves maturation of neuronal, glial and vascular cells, as well as cell proliferation and cell death. A fundamental understanding of cellular mechanisms is enhanced by quantification of cell types during key events in synapse formation and pruning and possessing qualified genetic tools for cell type-specific manipulation. Acquiring this information in turn requires validated cell markers and genetic tools. We quantified changing proportions of neurons, astrocytes, oligodendrocytes, and microglia in the medial nucleus of the trapezoid body (MNTB) during neural circuit development. Cell type-specific markers, light microscopy and 3D virtual reality software, the latter developed in our laboratory, were used to count cells within distinct cell populations at postna

SUBMITTER: Brandebura AN 

PROVIDER: S-EPMC9245928 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature