Determination of multiple drugs of abuse in human urine using dispersive liquid-liquid microextraction and capillary electrophoresis with PDA detection.

ABSTRACT: A new method was developed for pre-concentration and determination of multiple drugs of abuse in human urine using dispersive liquid-liquid microextraction (DLLME) and capillary electrophoresis (CE) with photodiode array detection. The method was based on the formation of tiny droplets of an organic extractant in the prepared sample solution using water-immiscible organic solvent (chloroform) dissolved in water-miscible organic dispersive solvent (isopropyl alcohol). The organic phase, which extracted eight drugs of abuse from the prepared urine solution, was separated by centrifugation. The sedimented phase was transferred into a small volume CE auto-sampler vial with 10 µL of 1% HCl methanol solution and evaporated to dryness. The residue was reconstituted in lidocaine hydrochloride (int

SUBMITTER: Meng L 

PROVIDER: S-EPMC9245984 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature