Functional Transcriptomic Studies of Immune Responses and Endotoxin Tolerance in Early Human Sepsis.

ABSTRACT:

Background

Limited studies have functionally evaluated the heterogeneity in early ex vivo immune responses during sepsis. Our aim was to characterize early sepsis ex vivo functional immune response heterogeneity by studying whole blood endotoxin responses and derive a transcriptional metric of ex vivo endotoxin response.

Methods

Blood collected within 24 h of hospital presentation from 40 septic patients was divided into two fractions and incubated with media (unstimulated) or endotoxin. Supernatants and cells were isolated, and responses measured using: supernatant cytokines, lung endothelial permeability after supernatant exposure, and RNA expression. A transcriptomic signature was derived in unstimulated cells to predict the ex vivo endotoxin response. The signature was t

SUBMITTER: Leligdowicz A 

PROVIDER: S-EPMC9246838 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature