Peptidoglycan compositional analysis of Mycobacterium smegmatis using high-resolution LC-MS.

ABSTRACT: Peptidoglycan (PG) is the exoskeleton of bacterial cells and is required for their viability, growth, and cell division. Unlike most bacteria, mycobacteria possess an atypical PG characterized by a high degree of unique linkages and chemical modifications which most likely serve as important determinants of virulence and pathogenesis in mycobacterial diseases. Despite this important role, the chemical composition and molecular architecture of mycobacterial PG have yet to be fully determined. Here we determined the chemical composition of PG from Mycobacterium smegmatis using high-resolution liquid chromatography-mass spectrometry. Purified cell walls from the stationary phase were digested with mutanolysin and compositional analysis was performed on 130 muropeptide ions that were identifie

SUBMITTER: Rimal B 

PROVIDER: S-EPMC9247062 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature